Ratiometric dating

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This range corresponds to the number of bits used for coding numbers in binary format. Thus, if a sensor used 8 bits to record the data, there would be 28=256 digital values available, ranging from 0 to 255.However, if only 4 bits were used, then only 24=16 values ranging from 0 to 15 would be available.The experiment requires two different fluorescent labels and only one of the two labels is bleached.It employs irradiation of a fluorophore in a living sample with a short laser to erase fluorescence followed by time-resolved image recording of the sample.

This illustration shows two hands juggle three balls representing radiometric, spectral and spatial resolution.Photobleaching experiments can be conducted with confocal laser scanning microscopes where the laser is used at high intensity for bleaching and low intensity for image recording.The most common way to fluorescently tag proteins in living cells is mutants EGFP is the best because of its high quantum yield, its low tendency of photobleaching as well as its relative photostability during post-bleach image acquisition.This demonstrates that the user must continuously make tradeoffs between these three resolutions...there are trade-offs between spatial, spectral, and radiometric resolution which must be taken into consideration when engineers design a sensor.

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    For example, you can’t find the remaining amount of an isotope as 7.5 half-lives by finding the midpoint between 7 and 8 half-lives.

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